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Substance P Regulates Macrophage Inflammatory Protein 3a /CCl20 with Heme Oxygenase-1 in Human Periodontal Ligament Cells KCI 등재

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대한구강악안면병리학회지 (The Korean Journal of Oral and Maxillofacial Pathology)
대한구강악안면병리학회 (Korean Academy Of Oral And Maxillofacial Pathology)
초록

Al t hough substance P(SP) , a potent pro- inflammatory peptide, is involved in inflammation and immune responses‘ t he eff'ect of SP on t he expression of macrophage inflammatory protein 3a (MIP- 3α CCL20) in periodontal liga ment(PDL) cell s a re unknown, Equally as enigmatic is the link between SP, t he stress protein heme oxygenase- l(HO-l) ‘ and CCL20 procluction, We investigated whether SP induces the release of chemokine CCL20 from immortal ized PDL(IPDL) ceJJ s‘ and fur ther c l a꺼 SP mediated pathways, We also examined the relationship between HO-l a ncl CCL20 by t reating PDL cells with SP, Incubating IPDL cells with SP increased expression of CCL20 mRNA a nd CCL20 protein in a dose-time dependent manner Highly selective p38 and ERKl/2 inhibitors abrogated SP-induced expression of CCL20 in IPDL cell s, SP is a lso responsible for ini t iating phosphorylation of I/C B, degradation of Iκ B‘ ancl activat ion of NF'-/C B, SP induced expression of HO-l in both a concentration- and time-dependent man nel ‘ and CCL20 refl ected s imilar patterns, The inductive effects o[ SP on HO- l and CCL20 wer e enhanced by HO- j inducer hemin and the membrane-permeable cGMP analog 8-bromo-cGMP, Conversely, this pathway was inJübited by t he 1-10난 inhi bitor zinc protoporphyrin IX(ZnPP IX) and the selective inl뼈itor of guanylate cyc1ase‘ lH-[l , 2, 4Joxad iazole[4‘ 3-aJquinoxal in-l-one (ODQ) , We report herein the pathway that connects SP along with other modulators 。f neuroimmunoregulationto the induction of HO-l and t he inflammatory mediator MIP-3a /CCL20 in IPDL cell s‘ which play an important role in the development 01' periodontitis or inflamrnation during orthodontic tooth movem

저자
  • Sun Kyung Lee(Department of Oral & Maxillofacial Pathology, College of Dentistry, Wonkwang University)
  • Sung Hee Pi(Department of Oral & Maxillofacial Pathology, College of Dentistry, Wonkwang University)
  • Sung Hyun Kim(Department of Oral & Maxillofacial Pathology, College of Dentistry, Wonkwang University)
  • Kyung San Min(Department of Oral & Maxillofacial Pathology, College of Dentistry, Wonkwang University)
  • Hwa Jeong Lee(Department of Oral & Maxillofacial Pathology, College of Dentistry, Wonkwang University)
  • Hoon Sang Chang(Department of Oral & Maxillofacial Pathology, College of Dentistry, Wonkwang University)
  • Kyung Hwa Kang(Department of Oral & Maxillofacial Pathology, College of Dentistry, Wonkwang University)
  • Hyung Ryong Kim(Department of Oral & Maxillofacial Pathology, College of Dentistry, Wonkwang University)
  • Hong ln Shin(Department of Oral & Maxillofacial Pathology, College of Dentistry, Wonkwang University)
  • Suk Keun Lee(Department of Oral & Maxillofacial Pathology, College of Dentistry, Wonkwang University)
  • Eun Cheol Kim(Department of Oral & Maxillofacial Pathology, College of Dentistry, Wonkwang University)