A novel indirubin analog, 5'-nitro-indirubinoxime inhibits cell proliferation and induces apoptosis against various human cancer cells. In this study, we performed the microarray analysis to identify genes differentially expressed in the KB oral squamous carcinoma cells after treated with 5'-nitro-indirubinoxime. Among the 10,800 genes analyzed, 1,701 genes (15.8%) showed statistically different expression level in the 5'-nitro-indirubinoxime treated cells with respect to untreated control cells. Among those, 263 genes (15.5%) were down-regulated and 220 genes (12.9%) were up-regulated more than 2-fold. Functionally related gene clusters include genes associated with signal transduction (18.1%), especially genes related with apoptosis (3.5%) and cell cycle regulation (5.8%). Our application of microarray analysis on 5'-nitro-indirubinoxime treated oral cancer cells allows the identification of candidate genes for providing novel insights into the indirubin mediated antitumor activity.

• 김수아(조선대학교 치과대학 구강병리학교실) | Soo A Kim
• 박고운(조선대학교 치과대학 구강병리학교실) | Go-Woon Park
• 김식(NURI Project, 구강악안면외과학교실) | Sik Kim
• 김수관(NURI Project, 구강악안면외과학교실) | Su Gwan Kim
• 김시욱(조선대학교 공과대학 환경공학부) | Si-Wook Kim
• 안상건(조선대학교 치과대학 구강병리학교실) | Sang-Gun Ahn
• 윤정훈(조선대학교 치과대학 구강병리학교실) | Jung Hoon Yoon Correspondence