rt is well known that glycosaminoglycans are 01' fllndamental importance to the processes 01' morphogenesis and cytodifferentiati on dllring the teeth development , With HlD-TCH-SP(High - iron diamine-thiocarbohydrazide-silvel protcinntc) , s lllfatcd glycosaminoglycans such as chondroitin sulfate and heparan slllfate have been localized a t the III trastrllctural level i n a wide variety of tlssues The pmpose of this stlldy were to examine slllfated glycosaminoglycan at llltrastrllctllral level fo 1' the phase of morphogenesis and cytodifferentiation of hllma n fetal tooth germs, and to detect the protein expression of slllfated glycosaminoglycan by immunoslot blot Human tooth gerll1s fl'Oll1 the a lveolar bone of twenty still born fetuses we1'e fixed in a mixtllre of 2% gllltaraldehyde/1% forma ldehyde, The 미 t 1'athin sections were stained witb HID-TCH-SP and were treated with 0, 05% solution of t esticular hyaluronidase to identify the histochemical properties 01' tbe HID-TCH-SP stain deposits , For semi quantitative protein assay , immllnoslot blot was done Sulfated glycocongugated deposits were localized in DEJ, peri tubular dentin , and mantle dentin matrix‘ enamel prism sheath , interrod area , and enamel matnx Heparan sll l띠 te deposits i n DEJ resisted to testicular hyalllronidase treatment prior to HID-TCH-SP staining, Immunoslot blot s howed that• chondroitin sulfate was detected higher in enamel and dentin extract, while heparan sulfate was relatively expressed in enamel and dentin extract, but rarely expressed in enamel or dentin extract It suggest ecl that chonclroi tin and hepa ran slllfate woulcl play an important role in the formation of DEJ, while chondroitin sulfate would in the clevelopll1ent of enamel prism sheath, enamel matrix, and mantle 01' peritublllar clentin of human fetal tooth germs,

• Gyeung Ju Park(Department of Oral Pathology, Dental College, Dankook University)
• Chong Heon Lee(Department of Oral Pathology, Dental College, Dankook University)