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RT-PCR을 이용한 구강 편평세포암종의 림프절 미세전이 검색 KCI 등재

Detection of Lymph Node Micrometastases of Oral Squamous Cell Carcinoma by RT-PCR

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대한구강악안면병리학회지 (The Korean Journal of Oral and Maxillofacial Pathology)
대한구강악안면병리학회 (Korean Academy Of Oral And Maxillofacial Pathology)
초록

Metastatic spread to cervical lymph nodes(LNs) is a major determinant of outcome in oral squamous cell carcinoma (SCC). To provide an useful method for the detection of lymph node micrometastases, we fulfilled the histopathological examination and reverse transcriptase polymerase chain reaction(RT-PCR) using the paraffin-embedded LNs of oral SCC patients. In this study, 78 LNs from 12 patients with primary oral SCC were analyzed. Metastases in the regional LNs were evaluated by RT-PCR for squamous cell carcinoma antigen(SCCA) and cytokeratin 5(CK5). Detectability of metastatic LNs by RT-PCR was compared with histopathological examination. Of 78 LNs, CK5 and SCCA mRNA were detected in 32(41.0%) and 8(10.3%), respectively. Histopathologically, 10(12.8%) of 78 LNs were positive. CK5 mRNA was detected in all 10 histopathologically positive LNs. In contrast, SCCA mRNA was detected in 5 of 10 histopathologically positive LNs. These findings suggest that genetic diagnosis by RT-PCR based on CK5 mRNA expression may be sensitive and clinically useful technique to detect the presence of metastatic carcinoma cells in regional lymph nodes of oral SCC.

저자
  • 문경숙(전북대학교 자연과학대학 생물과학부 전북대학교 치과대학 구강병리학교실 및 구강생체과학연구소) | Kyung Suk Moon
  • 김경숙(전북대학교 자연과학대학 생물과학부) | Kyung Suk Kim
  • 이승재(전북대학교 치과대학 구강병리학교실 및 구강생체과학연구소) | Seung Jae Lee
  • 송희정(전북대학교 치과대학 구강병리학교실 및 구강생체과학연구소) | Hee Jung Song
  • 최은정(전북대학교 치과대학 구강병리학교실 및 구강생체과학연구소) | Eun Jung Choi
  • 손현진(전북대학교 치과대학 구강병리학교실 및 구강생체과학연구소) | Hyun Jin Son
  • 조남표(전북대학교 치과대학 구강병리학교실 및 구강생체과학연구소) | Nam Pyo Cho