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Integrin 발현 차단이 구강암 세포주의 부착 및 침습능에 미치는 영향 KCI 등재

The effect of the blocking of integrin expression on the adhesion and invasion of oral cancer cell lines

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대한구강악안면병리학회지 (The Korean Journal of Oral and Maxillofacial Pathology)
대한구강악안면병리학회 (Korean Academy Of Oral And Maxillofacial Pathology)
초록

Alterations in cell surface receptors and adhesion molecules which regulate cell-cell and cell-matrix interactions have been 뻐plicated in tumor processes. In order to investigate the effect of integrin expression on the invasiveness of oral sqllamous cell carcinoma, integ띠1 expression in the celllines such as SCC-4, SCC-9, SCC-15, and SCC-25 was analyzed, and the comparison between cell adhesion assay towards extracellular matrix proteins and in vitro invasion assay following inhibition of the functional domain of the integrins using blocking antibodies against the specific integrins 낀 nd Arg-Gly-Asp (RGD) peptide were carried out. The expression of integrin a 2, a 3, a 6 was detected in all oral squamous cell carcinoma celllines. In contrast, the expression of a vß6 integrin is detected in SCC-4 and SCC-9, not in SCC-1 5 and SCC-25. 까1e adhesion of SCC-4 cell line to collagen 1, laminin, and fibronectin was significantly reducecl by σeatment with a 3-, a 6-, and a vß6-blocking antibody, respectively (p (0.05). 꺼.1e invasion of SCC-4 cell line throllgh Matrigel was significantly reduced by treatment with v 6-blocking antibody and RGD pepticle (p(0.05). These results sllggested that specifìc integrins play an in1portant role in the process of adhesion and invasion of oral squamous cell carcinoma cells and the expression of a vß6 integrin is believed to the enhance its invasivene잃.

저자
  • 임지준(서울대학교 치과대학 구강병리학교실) | Ji-Jun Lim
  • 홍성두(서울대학교 치과대학 구강병리학교실) | Seong-Doo Hong
  • 강민나(서울대학교 치과대학 구강병리학교실) | Min-Na Kang
  • 홍삼표(서울대학교 치과대학 구강병리학교실) | Sam-pyo Hong
  • 이재일(서울대학교 치과대학 구강병리학교실) | Jae-II Lee
  • 임창윤(서울대학교 치과대학 구강병리학교실) | Chang-Yun Lim