Porcine litter size is a quantitative trait and its heritability is especially low. So it is necessary to identify porcine reproductive gene and protein. The establishment of pregnancy requires performance of a receptive endometrium. We analyzed the endometrial tissue protein of porcine and would find out biomarker proteins related to porcine litter size. We sorted the two groups according to litter size of porcine: a small litter size group (SLSG) (n=2) and a large litter size group (LLSG) (n=2). The porcine endometrial tissue samples were analyzed separately using 2-dimensional electrophoresis (2-DE) within the isoelectric point ranges of 3.0 to 10.0, and then differential proteins were identified using MALDI-TOF analysis. In comparison of SLSG(small litter size group) with LLSG(large litter size group), a total of 9 protein spots differentially expressed on porcine endometrial tissue 2-DE gels, among which 5 spots were up-regulated proteins as retinol dehydrogenase 16-like isoform 1, Acrosin-binding protein, alpha-N-acetylgalactosaminidase. phosphoglycerate kinase 2, Acrosin-binding protein in LLSG. And 4 spots were up-regulated proteins as phosphoglycerate kinase 2, prenylcysteine oxidase in SLSG.

• Jae Eun Lee(Department of Animal Science & Biotechnology, Research Center for Transgenic Cloned Pigs, Chungnam National University)
• Tao Lin(Department of Animal Science & Biotechnology, Research Center for Transgenic Cloned Pigs, Chungnam National University)
• Reza K. Oqani(Department of Animal Science & Biotechnology, Research Center for Transgenic Cloned Pigs, Chungnam National University)
• Jung Won Kang(Department of Animal Science & Biotechnology, Research Center for Transgenic Cloned Pigs, Chungnam National University)
• So Yeon Kim(Department of Animal Science & Biotechnology, Research Center for Transgenic Cloned Pigs, Chungnam National University)
• Dong Il Jin(Department of Animal Science & Biotechnology, Research Center for Transgenic Cloned Pigs, Chungnam National University)