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Regulatory Roles of Oocyte Secreted Factors in Canine Cumulus-oocyte Complexes and Granulosa Cells

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한국동물생명공학회(구 한국수정란이식학회) (Journal of Animal Reproduction & Biotechnology)
초록

Oocyte is the central factor in the bi-directional communication axis in the ovarian follicles. It controls the cumulus or granulosa cells to perform functions which are beneficial for its own development via secreting paracrine growth factors, including GDF9 and BMP15. The aim of this study was to investigate whether the recombinant GDF9 and BMP15 are able to promote meiotic resumption and cumulus expansion of canine COCs during IVM, as well as to demonstrate the actions of GDF9 and BMP15 in regulating the expression of connexin transcripts in the ovarian granulosa cells. As results, GDF9 and BMP15 significantly improved the meiotic resumption rate and cumulus expansion by activating ERK1/2 signaling. Treatments with GDF9 significantly improved the expression of CyclinB1 but inhibited the expression of Cx43 transcripts. In addition, cumulus expansion genes (MAPK1, Ptgs2, Tnfaip6 and Ptx3) were differentially improved by GDF9 and BMP15. In the ovarian granulosa cells, GDF9 suppressed the expression of Cx43 transcripts by binding ALK4/5/7 receptors and activation Smad2/3 signaling, whereas, BMP15 stimulated the expression of Cx43 transcripts by binding ALK2/3/6 receptors and activating Smad1/5/8 signaling. In conclusion, by regulating functions of granulosa/cumulus cells, oocyte has the potential to enhance the growth and maturation of itself.

저자
  • Lili Zhuang(Division of Animal & Dairy science, College of Agriculture and Life Science, Chungnam National University)
  • Dong Eon Kim(Division of Animal & Dairy science, College of Agriculture and Life Science, Chungnam National University)
  • Chi Sun Yun(Division of Animal & Dairy science, College of Agriculture and Life Science, Chungnam National University)
  • Kuk bin Ji(Division of Animal & Dairy science, College of Agriculture and Life Science, Chungnam National University)
  • Eun Ji Lee(Division of Animal & Dairy science, College of Agriculture and Life Science, Chungnam National University)
  • Gyeong Yeob Kim(Division of Animal & Dairy science, College of Agriculture and Life Science, Chungnam National University)
  • Ji Hye Lee(Division of Animal & Dairy science, College of Agriculture and Life Science, Chungnam National University)
  • Min Kyu Kim(Division of Animal & Dairy science, College of Agriculture and Life Science, Chungnam National University)