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Developmental Competence and Reprogramming Efficiency of Bovine Cloned Embryos Produced by Two Cloning Methods

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한국동물생명공학회(구 한국수정란이식학회) (Journal of Animal Reproduction & Biotechnology)
초록

Bovine somatic cell nuclear transfer (bSCNT) embryos can develop to the blastocyst stage at a rate similar to that of embryos produced by in vitro fertilization (IVF). However, the efficiency of somatic cell cloning has remained low, and applications have been limited, irrespective of the nuclear donor species or cell types. One possible explanation is that the reprogramming factors of each oocyte is insufficient or not properly adapted for the receipt of a somatic cell nucleus, because it is naturally prepared only for the receipt of a gamete. Here, we would like to introduce the aggregation method (agSCNT), a new experimental system that enables and increase oocyte volume and examined its subsequent development. Judgement by the blastocyst formation rate or total cell number was significantly higher in the agSCNT group than that in the SCNT group, and was very similar to that in the control IVF group. Moreover, the cleavage formation rate in the agSCNT group (61.5 ± 1.3) was higher than that in the SCNT group (39.7 ± 2.1), while still less than that in the IVF group (75.4 ± 1.3). We also analyzed the epigenetic modifications in bovine IVF, agSCNT, and untreated SCNT embryos. In conclusion, the present study demonstrated that agSCNT improves the in vitro developmental competence and quality of cloned embryos, as evidenced by increased total cell numbers (TC).

저자
  • Il-Keun Kong(Department of Animal Science, Division of Applied Life Science (BK21 Plus) Institute of Agriculture and Life Science, Gyeongsang National University)
  • Imran Khan(Department of Animal Science, Division of Applied Life Science (BK21 Plus))
  • Shimin Zhang(Department of Animal Science, Division of Applied Life Science (BK21 Plus))
  • Myeong-Don Joo(Department of Animal Science, Division of Applied Life Science (BK21 Plus))
  • MMR Chowdhury(Department of Animal Science, Division of Applied Life Science (BK21 Plus))
  • Ayman Mesalam(Department of Animal Science, Division of Applied Life Science (BK21 Plus))
  • Kyeong-Lim Lee(Department of Animal Science, Division of Applied Life Science (BK21 Plus))
  • Seok-Hwan Song(Department of Animal Science, Division of Applied Life Science (BK21 Plus))
  • Lianguang Xu(Department of Animal Science, Division of Applied Life Science (BK21 Plus))