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Success of Embryo Transfer Depends on Tight Junction Biogenesis and Assembly of Blastocyst

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한국동물생명공학회(구 한국수정란이식학회) (Journal of Animal Reproduction & Biotechnology)
초록

Establishment of the Adherens junction (AJ) and Tight junction (TJ) are important steps in terms of morphological formation during preimplantation develoment. Particularly, TJ complex is crucial for cavitation in blastocyst. So far, many TJ protein/genes are revealed. However, the biological function and regulation of TJ were not elucidated during post implantation. We depleted several TJ and TJ associated genes using RNA interference, and examined preimplantation development with TJ. We tested functionality of paracellular sealing to determine integrity of TJ formation and examined TE differentiation indirectly using outgrowth assay in vitro. We observed defect of paracellular permeability in the TJ related genes knockdown(KD) blastocyst and abnormal outgrowth. Particularly, trophoblast cells were not stretched out in the KD groups. Finally, we did embryo transfer using the TJ genes KD and control blastocysts into surrogate mothers. We found lower of the implantation rates/ maintenance of pregnancy in the TJ KD groups (less than 40%) than in the controls (about 80%). In conclusion, TJ integrity is can be used as a selective marker for developmentally competent embryos and successful pregnancy.

저자
  • Inchul Choi(Department of Animal and Dairy Sciences, College of Agriculture and Life Sciences, Chungnam National University)
  • Jiyeon Jeong(Department of Animal and Dairy Sciences, College of Agriculture and Life Sciences, Chungnam National University)
  • Yelin Jeong(Department of Animal and Dairy Sciences, College of Agriculture and Life Sciences, Chungnam National University)
  • Jeeahn Lee(Department of Animal and Dairy Sciences, College of Agriculture and Life Sciences, Chungnam National University)