기능성 발효유로 알려진 케피어로부터 박테리오신 생산 유산균주(CJNU 2008)를 선발하였다. CJNU 2008 균주는 16S rRNA 유전자 염기서열 분석을 통해 Enterococcus faecium으로 동정되어 E. faecium CJNU 2008로 명명하였다. E. faecium CJNU 2008균주의 배양액 및 부분정제 농축액은 Listeria monocytogenes와 Staphylococcus aureus와 같은 병원성 세균을 포함하여 몇몇 균주들에 대해서 항균활성을 나타내었다. E. faecium CJNU 2008 균주의 박테리오신 생산을 위한 배양 최적조건은 MRS broth, 30℃ 및 pH 7.0으로 확인되었다.

A bacteriocin producing lactic acid bacterium was isolated from kefir which is functional fermented milk. The CJNU 2008 strain was identified as Enterococcus faecium by 16S rRNA gene sequence analysis and the strain named E. faecium CJNU 2008. Antibacterial spectrum of E. faecium CJNU 2008 has shown that several strains including pathogenic bacteria Listeria monocytogenes and Staphylococcus aureus were inhibited by the cell and culture supernatant. Optimal culture conditions for production of the bacteriocin from E. faecium CJNU 2008 were MRS broth as a medium, 30℃, and pH 7.0.

• 서숙진(한국교통대학교 생명공학과, Department of Biotechnology, Korea National University of Transportation) | Souk-Jin Seo
• 문기성(한국교통대학교 생명공학과, Department of Biotechnology, Korea National University of Transportation) | Gi-Seong Moon Corresponding author