논문 상세보기
pp.29-36
Background: Ganoderma lucidum cultured on hulled barley was investigated as a potential natural source of antioxidants and antiinflammatory agents.
Methods and Results: The yields from Ganoderma lucidum cultured on hulled barley water and ethanol extract were 17.69% and 25.77%, respectively. The antioxidant activity of Ganoderma lucidum cultured on hulled barley extracts was confirmed by various methods including assayss of 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2-azino-bis-(3-ethylbenzo thiazoline-6-sulfonic acid) (ABTS), nitrite radical scavenging, and Fe3+ to Fe2+ reducing power activity. The ethanol extract of Ganoderma lucidum cultured on hulled barley showed improved DPPH, ABTS and nitrite radical scavenging activity compared with the water extract. After treatment of RAW264.7 cells with Ganoderma lucidum cultured on hulled barley ethanol extracts, the cell viability compared with the control was 92.82%, even at a concentration of 3,000 ㎍/㎖. The ethanol extract inhibited reactive oxygen species (ROS) generation in RAW264.7 cells stimulated with H2O2, even at low concentrations. In addition, the ethanol extract showed an inhibitory effects on the production of lipopolysaccharide-induced nitric oxide (NO) in RAW264.7 cells.
Conclusions: This study suggests that the extract of Ganoderma lucidum cultured on hulled barley is a potential source of natural antioxidants and anti-inflammatory agents.
재료 및 방법
1. 실험 재료
2. 총 phenolic compound, flavonoid 및 ascorbic acid함량 측정
3. DPPH radical 소거활성 측정
4. ABTS radical 소거활성 측정
5. Nitrite 소거활성 측정
6. 환원력 측정
7. 세포 내 ROS 측정
8. 세포생존율 측정
9. NO 생성 평가
10. 통계분석
결과 및 고찰
1. 겉보리 영지버섯 추출물의 총 phenolic compound,flavonoid 및 ascorbic acid 함량
2. 겉보리 영지버섯 추출물의 DPPH radical, ABTS radical,nitrite 소거활성
3. 겉보리 영지버섯 추출물의 환원력
4. 겉보리 영지버섯 추출물의 H2O2로 유도된 세포 내 ROS생성 억제 효과
5. 겉보리 영지버섯 추출물의 LPS로 유도된 세포생존율과NO 생성 억제효과
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REFERENCES
