Background: To date, the anti-wrinkle efficacy of phellodendri cortex has not been defined. In this study, we investigated the nitric oxide (NO) production and elastase inhibitory activities of 80% methanol extract of Phellodendri cortex and its ethyl acetate fraction.
Methods and Results: We prepared 80% methanol extract, and its fractions from phellodendri cortex. The treatment of RAW 264.7 cell with 25㎍/㎖ 80% methanol extract and ethyl acetate fraction resulted in no toxicity. We conducted assays of nitric oxide (NO) production and elastase inhibition. In the NO production assay, the ethyl acetate fraction showed an inhibitory effect approximately 17 times stronger than the 80% methanol extract. In elastase inhibitory assay, the ethyl acetate fraction also showed a stronger effect than the 80% methanol extract. In order to standardize the extract and fraction, we used TLC to separate the extract and observed the plate under UV light. We confirmed that the known pharmacological ingredients berberine, and palmatine in the 80% methanol extract and the ethyl acetate fraction.
Conclusions: These results indicated that phellodendri cortex extract and its ethyl acetate fraction produced strong inhibitory effect on elastase and NO production.

서 언
 재료 및 방법
  1. 실험 재료
  2. 기기 및 시약
  3. 추출 및 분획
  4. 세포 배양
  5. Thin layer chromatography 전개
  6. 세포 생존율 측정
  7. Nitric Oxide 억제율 측정
  8. Elastase 저해 활성 측정
  9. 통계 처리
 결과 및 고찰
  1. 고형분 수율
  2. Thin layer chromatography 분석
  3. 세포 생존율
  4. Nitric oxide 생성 억제
  5. Elastase 저해 활성
 REFERENCES

• 엄지나(㈜지에프씨) | Ji Na Um Corresponding author
• 민진우(㈜지에프씨) | Jin Woo Min
• 주광식(㈜지에프씨) | Kwang Sik Joo
• 강희철(㈜지에프씨) | Hee Cheol Kang