Background : Extraction is the first and the most important step in the recovery and purification of bio-active compounds from plant materials. Many important factors such as solvent, solvent composition, solvent to solid ratio, pH, and temperature significantly influence the extraction efficiency of bio-active compounds. Factorial design of a limited set of variables is advantageous when compared to the conventional method which varies a single parameter per trial. Methods and Results : The objective of this study was to screen independent factors, namely, ethanol concentration (60 – 100%), extraction temperature (40 – 80℃), time (6 – 18 hours), and liquid to solid ratio (10 – 50 ㎖/g) on the recovery of the extract yield, antioxidant capacity, phenolic and flavonoid contents from Dendropanax morbifera leaf using factorial design. Total flavonoid content of extract was determined by colorimetric method with aluminum chloride, while antioxidant activity was screened using the DPPH radical scavenging activity, TEAC and FRAP assays. Full factorial design was employed to determine the significant contribution of the above factors towards antioxidant capacity (TEAC, DPPH and FRAP), and flavonoid contents. Among, all the factors examined, ethanol concentration and extraction temperature are very significant (p < 0.0001), in obtaining higher antioxidant activity, total flavonoid contents. Conclusion : Two level full factorial design screening was successfully employed to determine the significant factors, which are ethanol concentration, temperature, time and liquid to solid ratio in contributing to high antioxidant capacity (TEAC, DPPH and FRAP), and flavonoid content determination from Dendropanax morbifera leaf. From the results obtained, ethanol concentration and temperature was very significant (p < 0.0001), in obtaining higher antioxidant activity and flavonoid contents. Further work on optimization using these significant factors are in progress.

• Kyeoung Cheol Kim(Major in Plant Resource and Environment, SARI, Jeju National University) | 김경철
• Tae Kil Eom(Major in Plant Resource and Environment, SARI, Jeju National University) | 엄태길
• Ju Sung Kim(Major in Plant Resource and Environment, SARI, Jeju National University) | 김주성 Corresponding author