Background : Adenophora triphylla var. japonica (Regel) H. Hara shows vegetative growth by radical leaf until 1 year after sowing and shows reproductive growth during the second year and there is a characteristic of bolting by turning into cauline leaf. In addition, the phenotypes of plants varies even though they are belonging to the same species. For this reason, there is a limit for the classification of the species by the method of visual examination. Methods and Results : Simple sequence repeat (SSR) markers were developed based on the genomic sequence of A. triphylla using next generation sequencing to prepare the basis of molecular breeding and analyze the genetic diversity. Ninety-five primer sets including tri-, tetra- and penta-nucleotide motif types were randomly selected and they were applied to mixed genomic DNA and finally 39 primer sets showing from two to four bands were selected and used for genetic relationship analysis. Conclusions : Using the next generation sequencing, 39 polymorphic SSR markers were developed.

• Ki Chan Park(Department of Herbal Crop Research, NIHHS, RDA) | 박기찬
• Young Gug Kim(Department of Herbal Crop Research, NIHHS, RDA) | 김영국
• Kyoung Hwangbo(Department of Industrial Plant Science and Technology, Chungbuk National University) | 황보경
• Jinsu Gil(Department of Industrial Plant Science and Technology, Chungbuk National University) | 길진수
• Hee Chung(Department of Industrial Plant Science and Technology, Chungbuk National University) | 정 희
• Sin Gi Park(TheragenEtex Bio Institote, Theragen Etex) | 박신기
• Chang Pyo Hong(TheragenEtex Bio Institote, Theragen Etex) | 홍창표
• Yi Lee(Department of Industrial Plant Science and Technology, Chungbuk National University) | 이 이 Corresponding author