The purpose of this study is to look into the effect of a horticultural activity program on career women’s job satisfaction. As the research subject, this study set 37 career women of 6 occupational clusters, such as elementary school teachers, nurses, social workers, care teachers, and police officers in ‘P’ city, and financiers in ‘D’city. The horticultural activity program was applied for about 10 months from June 2013 to April 2014, and for each job group the program was executed once a week and a total of 8 sessions. As a result of the analysis of job satisfaction by conducting horticultural activity by 6 occupational cluster, occupational clusters like an elementary school teacher, and social worker showed a significant improvement in job satisfaction immediately after horticultural activity was conducted, and in 4 weeks after that, all 5 occupational clusters but a care teacher cluster were found not to show a significant difference. It is expected that various horticultural activity programs would provide emotional stability to working women and consequently improve their overall quality of life. As a result of the above research, this study confirmed that improvement in job satisfaction is more effective when a person steadily participates in gardening activity rather than doing gardening activity temporarily. In addition, this study confirmed that gardening activity could be an alternative remedy which provides a positive change to a career woman’s job satisfaction.