논문 상세보기

Improved Cold Tolerance by Transformation with soybean SCOF-1 Gene in Populus alba KCI 등재

  • 언어ENG
  • URLhttps://db.koreascholar.com/Article/Detail/327099
구독 기관 인증 시 무료 이용이 가능합니다. 4,200원
농업생명과학연구 (Journal of Agriculture & Life Science)
경상대학교 농업생명과학연구원 (Institute of Agriculture & Life Science, Gyeongsang National University)
초록

The introduction of stress up-regulated genes into heterologous plants is found to confer stability trait to plants in otherwise hostile environments. The soybean cold inducible factor-1 gene(SCOF-1) encoding a zinc finger transcription factor was introduced into Populus alba L.(white poplar) by co-culture with Agrobacterium tumefaciens GV3101 that carried the binary vector, pGA643/SCOF1. The putative P. alba transformed plants were selected on Murashige and Skoog medium supplemented with 2.22μM 6-benzylaminopurine, 1.18mM carbenicillin, and 28.47μM hygromycin. The integration of SCOF-1 into the P. alba genome was also established by polymerase chain reaction(PCR), and the expression of the gene was confirmed by reverse transcription PCR. After exposure for 4h at 4℃ followed by 48h recovery at 27℃, the transgenic plants completely recovered to normal growth status. The chlorophyll content in transgenic plants was higher than that in wild type plants after chilling treatment. However, the moisture content and total phenolics was also not significantly different in transgenic and wild type plants after cold treatment.

저자
  • Yong-Duck Kim Corresponding author