Vinca alkaloids from plant Vinca minor have been investigated for their effects of tyrosinase inhibition, stimulation of ROS generation and increasement of cell migration activity. The methanolic crude extract and the water-soluble fraction exhibited IC50 value of 3.1 mg/mL and 2.1 mg/mL. Vinca minor extract treatment significantly increased ROS levels in HaCaT cells, in a concentration-dependent manner. Treatments of Vinca minor extract led to increase wound closure when compared with non-treatment. Low dose (0.1% or 0.3%) of extracts have not significantly affected, compared with that in controls. By contrast, 0.5% extract have dramatic effect on wound healing activity of keratinocytes. Effects of Vinca minor extract in a filter-based cell mobility assay appear similar to that of wound closure assay, which suggests that the Vinca minor extract have wound healing effects on skin.

• Jun-Sub Kim(Department of Biotechnology, Korea National University of Transportation, 61 Daehak-ro, Jeungpyeong-gun, Chungbuk 368-701)
• Il-Hwan Yu(Department of Biotechnology, Korea National University of Transportation, 61 Daehak-ro, Jeungpyeong-gun, Chungbuk 368-701)
• Ji-Hye Joo(Department of Biotechnology, Korea National University of Transportation, 61 Daehak-ro, Jeungpyeong-gun, Chungbuk 368-701)
• Gyeong Hoe Nam(Department of Biotechnology, Korea National University of Transportation, 61 Daehak-ro, Jeungpyeong-gun, Chungbuk 368-701)
• Kyung-Hwan Jung(Department of Biotechnology, Korea National University of Transportation, 61 Daehak-ro, Jeungpyeong-gun, Chungbuk 368-701)
• Young Soo Chung(Beauty Cosmetic Co., Ltd. 274-14, Wonnamsandan-ro, Wonnam-myeon, Eumseong-gun, Chungbuk, Korea)
• Hyang-Yeol Lee(Department of Biotechnology, Korea National University of Transportation, 61 Daehak-ro, Jeungpyeong-gun, Chungbuk 368-701) Corresponding author