This study was conducted to investigate the antibacterial, antioxidant, and in vitro greenhouse gas mitigation activities of fermented Scutellaria baicalensis Georgi extract. Seven starter cultures were used, comprising four of lactic acid bacteria and three of Saccharomyces cerevisiae. Ten grams of S. baicalensis Georgi powder was diluted in 90 mL autoclaved MRS broth. Each seed culture was inoculated with 3-10% (v/v) S. baicalensis Georgi MRS broth and incubated at 30℃ for 48 h. Among the starter cultures used, only Lactobacillus plantarum EJ43 could withstand the fermentation conditions. This fermentation broth was dried and extracted with ethanol to assess its antibacterial, antioxidant, and in vitro methane mitigation activities. The extract of S. baicalensis Georgi fermented by L. plantarum EJ43 (SBLp) showed higher antibacterial activity (bigger clear zone) compared to the unfermented S. baicalensis Georgi extract (SB0). SBLp also presented 1.2 folds higher antioxidant activity than SB0. During in vitro rumen fermentation, SBLp showed reduction in methane production compared to SB0 or the control. In conclusion, fermentation by L. plantarum EJ43 may enhance antibacterial and antioxidant activities of S. baicalensis Georgi and decrease enteric methane production.

• Tabita Dameria Marbun(Department of Animal Science, Kyungpook National University, Sangju 37224, South Korea)
• Jaeyong Song(Department of Animal Science, Kyungpook National University, Sangju 37224, South Korea) | 송재용
• Kihwan Lee(Department of Animal Science, Kyungpook National University, Sangju 37224, South Korea) | 이기환
• Su Yeon Kim(Department of Animal Science, Kyungpook National University, Sangju 37224, South Korea) | 김수연
• Juhui Kang(Department of Animal Science, Kyungpook National University, Sangju 37224, South Korea) | 강주희
• Sang Moo Lee(Department of Animal Science, Kyungpook National University, Sangju 37224, South Korea) | 이상무
• Young Min Choi(Department of Animal Science, Kyungpook National University, Sangju 37224, South Korea) | 최영민
• Sangbuem Cho(CALSNBT Co. Ltd., 501, Building C 131, Gasan digital 1-ro, Guemcheon-gu, Seoul 08506, South Korea) | 조상범
• Guiseck Bae(Department of Animal Science and Technology, Chung-Ang University, Anseong, 17546, South Korea) | 배귀석
• Moon Baek Chang(Department of Animal Science and Technology, Chung-Ang University, Anseong, 17546, South Korea) | 장문백
• Eun Joong Kim(Department of Animal Science, Kyungpook National University, Sangju 37224, South Korea) | 김은중 Corresponding author