We aimed to assess the behavior characteristics of fish on an artificial reef using hydroacoustic techniques. The acoustical survey was conducted with a 200 kHz dual beam transducer while fishing on the stone combination reef of Uljin. A school of fish were detected on the artificial reef before sunset and floated on the artificial reef at 30 minutes after sunset. The density (Nautical Area Scattering Coefficient, NASC) of fish that floated on the artificial reef after sunset was about 600 m2/nmi2; similar observations (about 50 m2/nmi2 or less) were noted after 19:00 hours. Fish caught by fishing on the artificial reef were Sebastes schlegeli, Hexagrammos otakii, Sebastes thompsoni, and Conger myriaster. Resultantly, we demonstrated that hydroacoustic techniques are useful for detecting behavior characteristics of fish in the artificial reef. Such results can be used for basic data to estimate the install effect of the artificial reefs.