This study was conducted to evaluate effect of α-linolenic acid (ALA) and bovine serum albumin (BSA) on viability, acrosome reaction and mitochondrial intact in frozen-thawed boar sperm. The boar semen was collected by gloved-hand method and cryopreserved using freezing extender containing 3 ng/mL ALA and/or 20 μg/mL BSA. Cryopreserved boar sperms were thawed in 37°C water-bath for 45 sec to analysis. Viability, acrosome reaction, and mitochondrial intact were analyzed using flow cytometry. In results, viability of frozen-thawed boar sperm was significantly higher in only ALA+BSA supplement group than control group (p<0.05), whereas there was no difference either in ALA or BSA supplement. However, acrosome reacted sperm in both of live and all sperm population were significantly decreased in all treatment groups than control (p<0.05). Interestingly, mitochondrial intact of boar sperm was enhanced in ALA and ALA+BSA groups compared with control (p<0.05). In this study, we showed that supplementation of ALA and BSA in freezing extender enhanced the sperm viability, mitochondrial intact and decrease acrosomal membrane damage. In conclusion, our findings suggest that quality of frozen-thawed sperm in mammalians could improve by using of ALA and BSA.

• Won-Hee Lee(College of Animal Life Sciences, Kangwon National University, Chuncheon 24341, Republic of Korea)
• Yong Hwangbo(College of Animal Life Sciences, Kangwon National University, Chuncheon 24341, Republic of Korea)
• Sang-Hee Lee(Institute of Animal Resources, Kangwon National University, Chuncheon 24341, Republic of Korea)
• Hee-Tae Cheong(College of Veterinary Medicine, Kangwon National University, Chuncheon 24341, Republic of Korea)
• Boo-Keun Yang(College of Animal Life Sciences, Kangwon National University, Chuncheon 24341, Republic of Korea)
• Choon-Keun Park(College of Animal Life Sciences, Kangwon National University, Chuncheon 24341, Republic of Korea) Corresponding author