In order to establish the basic data for the active use of the LED light source in the aquaculture industry, the experiment about the behavior of rockfish (Sebastes inermis) corresponding to the LED light was conducted by image analysis. LED lights for the experiment were one red light (wave length: 622 nm; light power: 811 mW) and two green lights (wave length: 518 nm; light power: 648 mW, wave length: 518 nm; light power: 810 mW). Behavior of the rockfish was analyzed using a moving distance (MD5) for 5 minutes where MD5s during the period of feeding were 20.0 m, 19.6 m, 16.3 m and 20.5 m in the ch1, ch2, ch3 and ch4. At the sunrise, mean MD5 of the entire channel about right before and right after were 6.3 m and 8.2 m. At the sunrise, mean MD5s of the entire channel about right before and right after were 13.6 m and 12.0 m. In the ch1, ch2 and ch3, mean MD5s during the period of 24 hours were 13.2 m, 9.6 m and 6.8 m at experiment 1and 5.2 m, 3.8 m and 2.9 m in the experiment 2.