The attachment of 2-aminobenzamide to carboxylated multi-wall carbon nanotubes (MWCNTs)- COOH was achieved through the formation of amide bonds. Then, the functionalized MWCNTs, MWCNT-amide, were treated by phosphoryl chloride to produce MWCNT-quin. The products were characterized by Fourier transform infrared spectroscopy, Raman spectroscopy, scanning electron microscopy, thermogravimetric analysis, derivative thermogravimetric, steady-state fluorescence spectroscopy, and solubility testing. MWCNT-quin showed photo-electronic properties, which is due to the attachment of the 4-hydroxyquinazoline groups to them as proved by steady-state fluorescence spectroscopy. This suggests intramolecular interactions between the tubes and the attached 4-hydroxyquinazoline. The toxicity of the samples was evaluated in human embryonic kidney HEK293 and human breast cancer SKBR3 cell lines, and the viable cell numbers were measured by 3-(4,5-dimethyl- 2-thiazolyl)-2,5-diphenyltetrazolium bromide (MTT) after the cells were cultured for 24 h. Cellular investigations showed that the modified MWCNTs, particularly MWCNT- quin, have considerably significant toxic impact on SKBR3 as compared to HEK293 at the concentration of 5 μg/mL.

• Hasan Tahermansouri(Department of Chemistry, Ayatollah Amoli Branch, Islamic Azad University, Amol 678, Iran) Corresponding Author
• Atieh Mirosanloo(Department of Chemistry, Ayatollah Amoli Branch, Islamic Azad University, Amol 678, Iran)
• Saeed Heidari Keshel(Stem Cell Preparation Unit, Eye Research Center, Farabi Eye Hospital, Tehran University of Medical Sciences, Tehran 1336616351,Iran)
• Mossa Gardaneh(National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran 14965/161, Iran)