This paper studies the effect of a playtykurtic excitation signal on fatigue life. In general, a vibration test uses an excitation signal having a normal distribution of kurtosis 3. However, a vibration signal generated in a product’s operation environment has usually a non-normal distribution. This paper uses ZMNL method to generate a playtykurtic excitation signal having the equivalent power spectrum density with a reference vibration profile. This paper proposes a method which uses a fatigue damage spectrum ratio for studying the effect of a playtykurtic excitation signal on fatigue life. The fatigue spectrum damage ratio is obtained by dividing a fatigue damage spectrum acquired using the acceleration profile in which all conditions are the same except for the kurtosis by a fatigue damage spectrum acquired theoretically. This paper shows that the signal of kurtosis less than 3 can increase the fatigue damage spectrum by more than 2 to 4 times compared to the signal of kurtosis 3 using the fatigue damage spectrum ratio.