This study was intended to evaluate the removal efficiency of nutrients in effluents of wastewater using microalgae. Microalgae used in the culture experiment collected in stream and reservoir located in Gyeongsangbuk-do. Dominant species in prior-culture tank were Monoraphidium contortum, Scenedesmus acutus, Coelastrum microporum and Chlorella sp. Dominant species in synthetic wastewater culture under the 4000 Lux and 8000 Lux were Chlorella sp. and Scenedesmus obliquus. The removal efficiency of NO3-N under the 4000 Lux and 8000 Lux were 27.2%~88.1% and 63.0%~83.6% respectively. The removal efficiency of PO4-P under the 4000 Lux and 8000 Lux showed above 93%. Removal efficiency of nutrients of 1.0×106 cells mL-1 inoculation concentration was more higher than that of nutrients of 1.0×105 cells mL-1 and 1.0×107 cells mL-1 inoculation concentration. Microalgae cultured in synthetic wastewater removed 94.9% of TN and 90.0% of TP. The removal rate of TN and TP in synthetic wastewater were 1.961 mg L-1 day-1 and 0.200 mg L-1 day-1 respectively. Nutrient removal efficiency of microalgae according to kinds of wastewater showed the highest in the private sewage.