This study aims to analyze the changes in the mechanical properties of interlock fabrics knitted with three types of fibers (i.e., cotton, wool, and polyester) by bonding fusible interlinings with varying deniers (i.e., 10D, 20D, and 30D) for a 3D virtual try-on system. We experimented with four properties and thicknesses of twelve specimens of interlining bonded knitted fabrics including face fabrics and interlinings. The results showed that the tensile property changed values (i.e., LT increased, and WT and RT decreased) according to the denier of interlinings; however, the change was slight. On the other hand, the bending property increased significantly as the denier of the interlining increased on both the wale and the course. Among shearing properties, the value of G increased as the denier of the interlining increased on both the wale and the weft; however, 2HG decreased. Additionally, changes in the compression property varied according to the fibers and the denier of the interlinings. The thickness of the knitted fabrics increased or decreased slightly by bonding the interlining. based on these results, we conclude that the 3D virtual system users need to reflect these numerical changes of interlock fabrics by bonding interlinings when they perform fitting tasks on the screen to accurately express the to accurately express the manufacturing conditions of the real garment.