The final goal of this research is to develop a botanical biofiltration system, which combines green interior, biofiltering, and automatic irrigation, which can purify indoor air pollutants according to indoor space and the size of biofilter. The biofilter used in this experiment was designed as an integral form of water metering pump, water tank, blower, humidifier, and multi-level planting space in order to be more suitable for indoor space utilization. This study was performed to compare indoor air quality between the space adjacent to a botanical biofilter and the space away from the biofilter (control) without generation of artificial indoor air pollutants, and to evaluate plant growth depending on multiple floors within the biofilter. Each concentration of indoor air pollutants such as TVOCs, monoxide, and dioxide in the space treated with the biofilter was lower than that of control. Dracaena sanderiana ‘Vitoria’ and Epipremnum aureum ‘N Joy’ also showed normal growth responses regardless of multiple floors within the biofilter. Hence, it was confirmed that the wall-typed botanical biofilter suitable for indoor plants was effective for indoor air purification.