In the present study, we investigated the effect of 70% EtOH extract from Hippophae Rhamnoides L. leaves (HRL) on the anti-obesity effect in 3T3-L1 cells. The effects of HRL on lipid accumulation in 3T3-L1 cells were examined using Oil Red O staining. In addition, we examined the gene expression levels by using RT-PCR and western blot. The results of this analysis showed that 100 ㎍/㎖ HRL significantly increased the inhibition of lipid accumulation by 82.25%; significantly decreased the mRNA expression of sterol regulatory element binding protein-1c (SREBP-1c), peroxisome proliferator-activated receptor γ (PPARγ), CCAAT/enhancer binding proteins α (C/EBPα), and fatty acid synthase (FAS) in 3T3-L1 cells as well as the stimulated protein expression of AMP-activated protein kinase (AMPK); and suppressed the expression level of PPARγ. These results suggest that HRL can prevent adipogenesis through activation of AMPKα and inhibition of adipogenesis transcription factors.

• 정현주(삼성생약(주) 바이오생명공학연구소) | Hyeon Ju Jeong (Biotechnology institute, Samsung Herb Medicine Agricutural Co.)
• 박주희(삼성생약(주) 바이오생명공학연구소) | Ju Hee Park (Biotechnology institute, Samsung Herb Medicine Agricutural Co.)
• 김명조(강원대학교 식물자원응용과학과) | Myong-Jo Kim (Department of Applied Plant Sciences, Kangwon National University) Corresponding author