Pink Rot on melon and White Stain Symptom on grape are caused by Trichothecium roseum, one of the most important diseases of grape and melon. These diseases have been occurred in national-wide in Korea and causes irreversible damage on the grape and the melon at harvest season. This research presents the evaluation of the capacity of Bacillus subtillis HK2 to protect both melon and grape against T. reseum and establishes its role as a biocontrol agent. In this study, we isolated a Bacillus strain HK2 from rhizosphere soil, identified it as Bacillus subtillis by 16S rRNA analysis and demonstrated its antifungal activity against T. roseum. Under I-plate assay it was observed that the effect of hyphal growth inhibition was not due to production of volatile compounds. The optimum culture condition of HK2 was found at 30℃ and initial pH of 7.0. Application of HK2 culture suspension reduced 90.2% of white stain symptom on grape as compared to control, resulting in greater protection to grape against T. roseum infestation. Butanol extract of HK2 culture purified using flash column chromatography. The antifungal material was a polar substance as it showed antifungal activity in polar elute. Therefore, our results indicated a clear potential of B. subtilis HK2 to be used for biocontrol of Pink rot in melon and white stain symptom on grape caused by T. roseum.

• 오소영(국립한경대학교 국제농업기술정보연구소) | Soh-Young Oh (Research Institute of International Agriculture, Technology and Information, Hankyong National University)
• 이은영(국립한경대학교 원예학과) | En-Young Lee (Department of Horticulture, Hankyong National University)
• 남기웅(국립한경대학교 원예학과) | Ki-Woong Nam (Department of Horticulture, Hankyong National University)
• 윤덕훈(국립한경대학교 국제농업기술정보연구소) | Deok-Hoon Yoon (Research Institute of International Agriculture, Technology and Information, Hankyong National University) Corresponding author