A karyotype analysis of five wild rose species (Rosa helenae, R. mulliganii, R. multiflora, R. rubus, and R. solieana) was performed using bicolor fluorescence in situ hybridization (FISH). All five species were found to be diploid (2n = 2x = 14). The chromosome length in the metaphase stage ranged from 1.29 to 2.05 μm in R. helenae, 3.32 to 6.82 μm in R. mulliganii, 1.58 to 2.24 μm in R. multiflora, 2.05 to 3.46 μm in R. rubus, and 1.62 to 2.46 μm in R. solieana. The chromosomes were either metacentric or submetacentric, with no subtelocentric or telocentric chromosomes being observed. Each p air of 5 S and 4 5S rDNA sites was detected at the proximal region of the long arm and the terminal region of the short arm of chromosome #7, respectively.

• Yoon-Jung Hwang(1Department of Convergence Science, Sahmyook University, Seoul 01795, Korea 2Department of Environmental Horticulture, Sahmyook University, Seoul 01795, Korea)
• Yoon Ha Ju(Department of Convergence Science, Sahmyook University)
• Franklin Hinosa Mancia(Department of Environmental Horticulture, Sahmyook University)
• Yun-Im Kang(Department of Horticulture Science, Chonnam National University)
• Tae Ho Han(Department of Horticulture Science, Chonnam National University)
• Sung Mi Kim(Department of Environmental Horticulture, Sahmyook University)
• Ki-Byung Lim(Department of Horticultural Science, Kyungpook National University) Corresponding author