논문 상세보기
pp.631-642
식물 및 동물성 단백질 유래 펩타이드 형태의 단백질 가수 분해물들은 항산화, 고혈압 완화, 면역조절, 진통완화 및 항균작용 등 생리활성이 있는 것으로 알려져 왔다. 본 연구는 6가지 프로티아제 를 이용하여 오계란 단백질 가수분해물을 생산하고, 생산된 펩타이드의 항산화 능력을 평가하였다. 그 결과 가수분해도의 최대값은 protamex(46.3%)이고, DPPH 라디칼 소거능 최대값은 bromelain(57.23%), 하이드록시 라디칼 소거능 최대값은 alcalase(30.21%), 슈퍼옥사이드 라디칼 소거능 최대값은 alcalase(58.07%), Fe2+ 킬레이션 능력 최대값은 alcalase(72.06%)로 나타났다. 더 나아가 효소별 항산화 저해 능력 IC50 평가하였다. 그 결과 alcalase에 의한 최대값은 금속 킬레이션 눙력(IC50, 1.24 mg/mL) 이고, bromelain에 의한 최대값은 DPPH 소거능(IC50, 2.46 mg/mL)이고, flavourzyme에 의한 최대값은 금속 킬레이션 능력(IC50, 1.25 mg/mL)이고, neutrase에 의한 최대값은 DPPH 소거능(IC50, 3.64 mg/mL)이고, papain에 의한 최대값은 DPPH 소거능(IC50, 3.82 mg/mL)이고, protamex에 의한 최대값 은 DPPH 소거능(IC50, 1.93 mg/mL)이었다. 따라서 protease를 이용하여 오계란 단백질에서 추출한 펩 타이드는 항산화 기능성 식품소재로서 활용할 가치가 높을 것으로 기대한다.
Protein hydrolysates derived from plants and animals having antioxidant, suppression of hypertension, immunodulatory, alleviation of pain, and antimicrobial activity has been known as playing important role like hormone. This study was performed to hydrolysis of Ogae egg white protein using the six proteases. The antioxidant activity of the produced peptides was analyzed. As a result, the maximum value of hydrolysis was protamex(46.3%), DPPH radical scavenging was bromelain(57.23%), hydroxy radical scavenging was alcalase(30.21%), superoxide radical scavenging was alcalase(58.07%), and Fe2+ chelation ability was alcalase(72.06%). Furthermore, the antioxidant Inhibition concentration (IC50) of peptides was evaluated for each enzyme. As a result, the maximum value of alcalase was Fe2+ cheating ability(IC50, 1.24 mg/mL), bromelain was DPPH radical scavenging(IC50, 2.46 mg/mL), flavourzyme was Fe2+cheating ability(IC50, 1.25 mg/mL), neutrase was DPPH radical scavenging(IC50, 3.64 mg/mL), papain was DPPH radical scavenging (IC50, 3.82 mg/mL) and protamex was DPPH radical scavenging(IC50, 1.93 mg/mL). Therefore, we expect that peptides produced from Ogae egg white protein using protease enzyme are useful as an antioxidant functional food ingredients.
