The objective of this study was to evaluate the effect of the Allomyrina dichotoma larva (AD) on allergy and inflammation.We examined inhibitory effect of AD on allergic reactions in mast cells (RBL-2H3) activated by Compound 48 / 80and inflammatory response in macrophages (Raw 264.7) activated by LPS. Anti-allergy and anti-inflammatory actions ofAD water extract had no cytotoxicity. At these concentrations AD inhibited ẞ-hexosaminidase, tumor necrosis factor- α(TNF- α), interleukin-4 (IL-4), interleukin-6(IL-6) and cyclooxygenase-2(COX-2). AD also inhibited the expression of inducibleNO synthase (iNOS). AD reduced the release of inflammatory cytokines including IL-4, IL-6, TNF-α, and ẞ-hexosaminidase.These results suggest that AD may be potential anti-allergy and anti-inflammatory therapeutic agent.

• Hwa Jeong Lee(Department of Agricultural Biology, National Institute of Agricultural Sciences)
• Minchul Seo(Department of Agricultural Biology, National Institute of Agricultural Sciences)
• In-Woo Kim(Department of Agricultural Biology, National Institute of Agricultural Sciences)
• Joon Ha Lee(Department of Agricultural Biology, National Institute of Agricultural Sciences)
• Jae-Sam Hwang(Department of Agricultural Biology, National Institute of Agricultural Sciences)
• Mi-Ae Kim(Department of Agricultural Biology, National Institute of Agricultural Sciences)