Most insects possess two different acetylcholinesterases (AChEs) loci (ace1 and ace2). In contrast, cyclorrhaphan flies,including Drosophila flies, are known to have only a single ace2 locus according to the previous studies. However, sequenceinformation of two different ace genes of Drosophila suzukii have been reported in GenBank. To identify two ace genes(Dsace-A and Dsace-B) in D. suzukii, therefore, we extracted total RNA from 6 individuals and two ace genes successfullywere amplified using gene-specific primers, suggesting that two Dsace genes are transcribed. According to the amino acidsequence analysis, DsAChE-A possesses typical amino acids for 3D structure and catalytic activity of AChE, while someamino acid residues were not conserved in DsAChE-B, suggesting that DsAChE-A and DsAChE-B may be respectivetrue gene and pseudogene of two ace in D. suzukii.

• Yi Seul Kim(Department of Leisure Sports, Kyungpook National University)
• Young Ho Kim(Department of Applied Biology, Kyungpook National University)