Bemisia tabaci is a species complex consisting of at least 40 species which are morphologically indistinguishable. Althoughgenetic diversity of B. tabaci have been studied in many countries, its information is lack in Bangladesh. The COI sequencewas determined from 91 samples collected in Bangladesh and the phylogenetic relationship was constructed. Four crypticspecies (Asia I, Asia II-1, Asia II-5 and Asia II-10) were present in different regions. While both Asia I and Asia II-1was widely distributed in all around the country, Asia II-5 was central and southern regions, and Asia II-10 was onlyin the central region. Otherwise, our results showed that Asia II-5 had the highest haplotype and nucleotide diversity.Genetic groups of B. tabaci in Bangladesh were most similar with those of Thailand, Vietnam, Pakistan, China, and Indiabut did not show any aggressive cryptic species such as MED and MEAM-1.