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Tracing origin of oriental fruit fly Bactrocera doralis sp. complex(Diptera: Tephritidae) based on DNA barcoding analysis

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  • URLhttps://db.koreascholar.com/Article/Detail/334498
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한국응용곤충학회 (Korean Society Of Applied Entomology)
초록

The objectives of this study are to examine the genetic variation and the origin elucidation in oriental fruit flies (Diptera:Tephritidae) using DNA barcode, in prepartion for their future introductions into Korea. About 1,600 specimens of B.dorsalis sp. complex and B. correcta were collected from 10 countries, the Indochina peninsula, the Philippines, Taiwanand South China. A total of 182 cytochrome c oxidase I (COI) sequences were obtained and aligned from these regionalspecimens. Three chinese sequences from the GenBank reference were also included. Six hundreds base pair fragmentswere aligned and trimmed and used for a barcode. The phylogenetic tree was generated using the neighbor-joining methodwith 1,000 bootstrap replicates. There were two distinct groups in the phylogenetic tree, Bactrocera dorsalis sp. complexand B. correcta. Three specimen, intercepted in the hand-carried mango at the airport inspection and collected in the confiscatedmango, smuggled from Vietnam were included for a test. The DNA sequences from the airport were 100% identical toone of various Vietnam specimens, and that from the confiscated mango was mostly similar to those of Vietnam, suggestingthe usefulness of the barcode for elucidating the origin of oriental fruit fly.

저자
  • Sohee Kim(Department of Biology, Kunsan National University)
  • Seong-Jin Lee(Animal & Plant Quarantine Agency)
  • Heung-Sik Lee(Animal & Plant Quarantine Agency)
  • Jong-Ho Lee(Animal & Plant Quarantine Agency)
  • Hyojoong Kim(Department of Biology, Kunsan National University)