Actinobacteria have been known to produce variety of bioactive metabolites that include antibiotics and plant growthfactor. Also, Insecticidal compounds such as avermectin and tetranectin have been reported from actinobacteria. In thisstudy, to identify novel insect growth regulator (IGR)-based insecticidal compounds, 1,283 actinobacteria isolates weretested for their juvenile hormone antagonist (JHAN) activity using the yeast-two hybrid β-galactosidase assay. Amongthem, 25 isolates showed high level of JHAN activity and insecticidal activity against 3rd larvae of diamondback moth,Plutella xylostella. These results suggested that actinobacteria could be unparalleled source of novel IGR-based compounds.

• Bo Ram Lee(Department of Agricultural Biotechnology, Seoul National University)
• Jae Young Choi(Research Institute for Agriculture and Life Sciences, Seoul National University)
• Jong Hoon Kim(Department of Agricultural Biotechnology, Seoul National University)
• Dong Hwan Park(Department of Agricultural Biotechnology, Seoul National University)
• Seok-Hee Lee(Department of Agricultural Biotechnology, Seoul National University)
• Ying Fang(Department of Agricultural Biotechnology, Seoul National University)
• Min Gu Park(Department of Agricultural Biotechnology, Seoul National University)
• Ra Mi Woo(Department of Agricultural Biotechnology, Seoul National University)
• Woo Jin Kim(Department of Agricultural Biotechnology, Seoul National University)
• Yeon Ho Je(Department of Agricultural Biotechnology, Seoul National University)