For high-quality colored rice production, the cultivation environment is a critical factor. The major environmental factor is temperature, which includes the accumulated and average temperature during vegetative and reproductive stages. Generally, during the cultivation period, the temperature can be controlled by shifting the transplanting date. This study was carried out to determine the optimum transplanting date for high-quality red-colored rice production. Four red-colored rice varieties (Jeokjinju, Jeokjinjuchal, Hongjinju, and Gunganghongmi) were used as test materials. The transplanting dates were May 20 and June 5, 20, and 30 in 2015~2016. The most variable factor controlled by the transplanting date was the grain filling rate. The varieties transplanted on June 30 showed low yields owing to the decrease in the grain filling rate. In contrast, the polyphenol content increased with increasing delay in the transplanting date. Collectively, these two results indicate that the optimum transplanting date was June 20. The average temperature for 30 days after the heading date (30DAH) highly affected the polyphenol content. A lower temperature during the 30DAH induced higher polyphenol contents but also caused low yield. The optimum 30DAH temperature for obtaining a higher yield and polyphenol content was 22~23°C. Using the average 30DAH and accumulated temperatures, the optimum transplanting date was calculated as June 18 to 24 in Miryang region. The optimum transplanting date of Kyeungsangnamdo region was approximately mid-June to early July, and that of Kyeungsangbukdo region was approximately early to mid-June.