The purpose of this study was to investigate the effects of squat exercises with an upper limb resistance movement on the vastus medialis oblique, vastus lateralis, and gluteus maximus. This study recruited 16 healthy men. All subjects performed squat exercises with and without upper Limb resistance movements by using elastic tubing band. Electromyography (EMG) activity of the vastus medialis oblique, vastus lateralis, and gluteus maximus was recorded using a surface EMG system during these isometric squat exercises in both experimental conditions. The different muscle activation of the vastus medialis oblique, vastus lateralis, and gluteus maximus between the squat exercises with and without the upper limb resistance movement was analyzed using a paired t-test or Wilcoxon signed rank test. Our findings showed that the vastus medialis oblique, vastus medialis oblique/vastus lateralis ratio and gluteus maximus muscle activity was significantly increased during the squat exercise with upper limb resistance movements, as compared to the squat exercise without this component (p < .05). Based on our findings, the additional upper limb resistance movement may be a useful strategy to facilitate the vastus medialis oblique and gluteus maximus muscle activation during a squat exercise.