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Effect of DNA Vaccination with Retroviral Vector Expressing nfa1 Gene in Naegleria fowleri Infection KCI 등재

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농업생명과학연구 (Journal of Agriculture & Life Science)
경상대학교 농업생명과학연구원 (Institute of Agriculture & Life Science, Gyeongsang National University)
초록

Naegleria fowleri is pathogenic free-living amoeba leading to primary amoebic meningoencephalitis(PAM) in human and animals. The nfa1 gene cloned from N. fowleri is located on food-cup structure called pseudopodia and function an adherence of host target cells. To evaluate the effect of nfa1 vaccination against N. fowleri infection, we constructed retroviral vector(pQCXIN) expressing nfa1 gene. To determine the effect of vaccination and protective immunity in in vivo models, we measured the immunoglobulin levels, cytokine induction, and survival rate in mice infected with N. fowleri. Both levels of IgG and IgG subclass in DNA vaccinated mice were significantly elevated. The cytokine analysis show that DNA vaccinated mice induces production of IL-4 and IFN-γcytokines suggesting a Th1/Th2 mixed type immune response. The levels of nfa1 specific IgG antibody were maintained highly until 12 weeks post-vaccination in vaccinted mice. The nfa1 vaccinated mice using retroviral vector increased significantly survival rate(60%) after N. fowleri infection. Consequently, the nfa1 vaccination effectively induces protective immunity by upregulation of immune response in mice infected with N. fowleri. These results suggest that DNA vaccination using retroviral vector may be proper trial for treatment and prevention of PAM.

목차
ABSTRACT
 Introduction
 Materials and Methods
  1 Cultivation of Naegleria fowleri
  2 Expression and purification of recombinant Nfa1protein
  3 Retroviral vector construction and viral particlescollection
  4 DNA vaccination in mice
  5 Immunoglobulin analysis
  6 Cytokine analysis
  7 Infection of N. fowleri trophozoites and survivalrate of mice
  8 Statistical analysis
 Results and Discussion
 Acknowledgement
 References
저자
  • Changgeun Kang(Institute of Agriculture and Life Science, College of Veterinary Medicine, Gyeongsang National University)
  • Mehrnoosh kianpour(Institute of Agriculture and Life Science, College of Veterinary Medicine, Gyeongsang National University)
  • Il-Hwa Hong(Institute of Agriculture and Life Science, College of Veterinary Medicine, Gyeongsang National University)
  • Hae-Jin Sohn(Department of Microbiology, Ajou University School of Medicine)
  • Ho-Joon Shin(Department of Microbiology, Ajou University School of Medicine)
  • Jong-Hyun Kim(Institute of Agriculture and Life Science, College of Veterinary Medicine, Gyeongsang National University) Corresponding author