Soybean cultivars with genetically low levels of stachyose enhance the utilization of soybean in food as well as feed uses. The objective of this research is to obtain the information on indirection selection of soybean lines with low stachyose content using DNA marker based on RS2 (rs2) gene. Two genetic populations were developed from the crosses of three parents (116-13 parent : low stachyose content, PI417227 and PI506903 parents: normal stachyose content). Twenty F2 plants of RS2_ genotype and twenty F2 plants of rs2rs2 genotype from each populations were harvested. Content of stachyose was detected by HPLC. Stachyose contents (g/kg) of 116-13, PI417227, PI506903 parents were 3.7, 23.7, and 17.8, respectively. In population 1, stachyose content 20 F2 plants with RS2_ genotype was 14.8 – 24.1 and stachyose content 20 F2 plants with rs2rs2 genotype was 2.1 – 4.7. In population 2, stachyose content 20 F2 plants with RS2_ genotype was 12.4 – 19.7 and stachyose content 20 F2 plants with rs2rs2 genotype was 2.1 – 5.0. Mean difference between RS2_ genotype and rs2rs2 genotype in population 1 and 2 was highly significant. From this results, selection of genetic lines with low stachyose content by DNA marker based on RS2 (rs2) gene will be possible.

Abstract
 서 언
 재료 및 방법
  유전집단
  RS2 유전자형 분석
  Stachyose 함량 분석
 결과 및 고찰
 적 요
 사 사
 REFERENCES

• 최상우(경상대학교 생명과학연구원, 농학과, Department of Agronomy, Research Institute of Life Sci., Gyeongsang National University) | Sang Woo Choi
• 한성진(경상대학교 생명과학연구원, 농학과, Department of Agronomy, Research Institute of Life Sci., Gyeongsang National University) | Sung Jin Han
• 강동휘(경상대학교 생명과학연구원, 농학과, Department of Agronomy, Research Institute of Life Sci., Gyeongsang National University) | Dong Hui Kang
• 김진아(경상대학교 생명과학연구원, 농학과, Department of Agronomy, Research Institute of Life Sci., Gyeongsang National University) | Jin A Kim
• 이수진(경상대학교 생명과학연구원, 농학과, Department of Agronomy, Research Institute of Life Sci., Gyeongsang National University) | Su Jin Lee
• 정종일(경상대학교 생명과학연구원, 농학과, Department of Agronomy, Research Institute of Life Sci., Gyeongsang National University) | Jong Il Chung Corresponding Author