돌나물(Sedum sarmentosum)의 기능성과 화장품 소재로서의 특성을 파악하기 위하여 본 연구를 수행 하였다. 돌나물을 동결건조하고 분쇄하여 에탄올로 추출한 후 -20 ℃에 저장하면서 실험에 사용하였다. 돌나물 의 에탄올 추출물(SE) 내에 포함된 총폴리페놀화합물(TPC)의 함량은 27.98 ± 0.34 g/kg (건조중량)이었고, epicatechin (162.14 ± 5.07 mg/kg), epigallocatechin (55.99 ± 2.49 mg/kg) 및 kaempferol (47.96 ± 3.02 mg/kg)순으로 많이 함유되어 있는 것으로 분석되었다. SE는 organic radical 소거효과(78.43 ± 1.08%) 와 금속이온환원능(FRAP value 2.54 ± 0.12)을 나타내었다. 또한 SE는 지질과산화 반응의 초기 단계(FTC assay 62.03 ± 0.38%)와 최종단계(TBARS assay 55.36 ± 2.05%)를 저해하였다. SE (5 mg/mL 건조중 량)는 Propionibacterium acne에 대해 항균효과를 나타내었다. SE의 elastase 저해율은 38.94 ± 7.09%이었 고, collagenase 저해율은 78.94 ± 2.49%이었다. SE 처리구는 Col3A1 발현양이 증가하였고 collagen 생성율 은 대조구보다 58.11 ± 1.07% 높았다. 0.5% SE가 함유된 수중유적형 크림은 pH 6.88, 밀도 1.47 g/mL이었 고, 다양한 온도(-20 - 45 ℃)에 4주간 저장하는 동안 크림은 안정한 유화상태를 유지하였다. 따라서 SE는 항균, 항산화, 항노화 활성을 지닌 것으로 사료되었다.
This study was performed to investigate the functional properties and characteristics of Dolnamul (Sedum sarmentosum)
as a cosmetic ingredient. Lyophilized sedum powder was extracted with ethanol and stored at -20 ℃ for
the following experiments. Total polyphenol compounds of the ethanol extract of sedum (SE) was 27.98 ± 0.34 g/kg
(dry weight): epicatechin (162.14 ± 5.07 mg/kg), epigallocatechin (55.99 ± 2.49 mg/kg), and kaempferol (47.96 ± 3.02
mg/kg) were contained in the SE. The SE had organic radical scavenging capacity (78.43 ± 1.08%) and metal reducing
power (FRAP value 2.54 ± 0.12). FTC and TBARS assays confirmed that the SE inhibited the early stage of lipid peroxidation
(62.03 ± 0.38%) as well as the final stage of lipid peroxidation (55.36 ± 2.05%), respectively. The SE (5 mg/mL,
dry weight) was proved to have antibacterial effect on the growth of Propionibacterium acnes. The inhibitory percentages
of the SE on elastase and collagenase activities were 38.94 ± 7.09% and 78.94 ± 2.49%, respectively. Compare
to the control group, the SE treated group induced an increase of Col3A1 expression and collagen production (58.11
± 1.07%). The oil in water emulsion (0.5% SE adding group) showed pH 6.88 and 1.47 g/mL of density. The hardness
changes of the SE adding emulsions were not detected during the stored periods at various temperatures (-20 - 45 ℃)
for four weeks. It is considered that the SE has antibacterial, antioxidant, and antiaging activities.