In Korea, 1,212 accessions of garlic are conserved in Namhae, Jeonju, Danyang and Muan with IT (Identity) number. This study was carried out to evaluate characteristics of 163 garlic cultivars conserved in Namhae branch, NIHHS, RDA and search valuable genetic resources for breeding. In horticultural characteristics, there was wide variation for bulb weight, clove weight and clove number per bulb with large coefficient of variation (CV) 40.04%, 43.98% and 39.64% respectively. Alliin content analyzed by HPLC was 16.75±6.22mg·g-1 DW (Dry weight) and had 37.16% CV. The highest alliin content was 32.27mg·g-1 in Local5. In correlation analysis, there were a strong positive correlation between bulb weight and bulb diameter and a non-correlation between clove number per bulb and bulb weight, height and diameter. Major characters included in the principal components were bulb weight, bulb height, bulb diameter, plant height, clove number per bulb, leaf sheath diameter and alliin content. According to characteristics of garlic, 163 germplasms were clustered into 10 groups. In total 10 clusters, the average bulb weight is the heaviest at 5th cluster and average alliin content is the highest at 4th cluster. These clusters will be used for selection breeding program to cultivate garlic that has heavier weight and high alliin content.