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Reactive oxygen species (ROS) induces distinct gene expression in human fibroblast like synoviocytes (HFLS) KCI 등재

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  • URLhttps://db.koreascholar.com/Article/Detail/339176
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충북대학교 동물의학연구소 (Research Institute of Veterinary Medicine, Chungbuk National University)
초록

Adhesive capsulitis of the shoulder is a common cause of pain that occurs during shoulder movement, thereby restricting shoulder rotation in clinical practice. Although most patients respond to pain relief treatment (NSAID or corticosteroids) by improving their range of motion, it remains poorly understood without any definitive treatment algorithm. In addition to immune cells, synoviocytes, chondrocytes and osteoblasts in the joint are known to produce pro-inflammatory mediators such as reactive oxygen species (ROS), inflammatory cytokines and lipid mediators, presumably contributing to the pathogenesis of osteoarthritis (OA) and adhesive capsulitis. Although inflammation and also fibrosis are proposed to be the basic pathological changes of a frozen shoulder, there is a lack of information regarding the downstream targets of the pro-inflammatory ROS signaling pathway in the synoviocytes and also how these ROS targets are modulated at the transcription level by a corticosteroid - dexamethasone. In this study, we used human fibroblast like synoviocytes (HFLS) to characterize the signaling targets of ROS by employing a human DNA microarray tool and studied the role of dexamethasone in this process. Our data suggest that several genes such as FOS, FOSB and NFkBIZ, which are known to be involved in pro- or anti- inflammation response, are modulated at the transcription level by ROS and dexamethasone.

목차
Introduction
 Materials and Methods
  Cell culture and reagents
  DNA microarray
  Reversre Transcriptase (RT) PCR
 Results
 Discussion
 Acknowledgements
 Reference
저자
  • Kyung Min Kil(Department of Orthopaedic Surgery, College of Medicine, Chungbuk National University)
  • So-Hee Jung(Department of Biochemistry, College of Medicine, Chungbuk National University)
  • In-Sook Jeon(Department of Biochemistry, College of Medicine, Chungbuk National University)
  • Byung-Ki Cho(Department of Orthopaedic Surgery, College of Medicine, Chungbuk National University)
  • Ji Kang Park(Department of Orthopaedic Surgery, College of Medicine, Chungbuk National University)
  • Kyung-Jin Park(Department of Orthopaedic Surgery, College of Medicine, Chungbuk National University)
  • Yong-Min Kim(Department of Orthopaedic Surgery, College of Medicine, Chungbuk National University) Corresponding author
  • Joong Kook Choi(Department of Biochemistry, College of Medicine, Chungbuk National University) Corresponding author