논문 상세보기
pp.367-374
Background: An imbalance in energy intake and expenditure can cause obesity, which is a major risk factor for chronic diseases such as heart disease, type 2 diabetes, insulin resistance, cancers and hyperlipidemia. Methods and Results: In this study, we evaluated the anti-obesity effects of a water extract from the young leaves of barley sprout (BS) in 3T3-L1 cells and in high-fat diet (HFD)-induced obese mice (HF). Lipid accumulation measurement indicates that BS markedly inhibited adipogenesis by reducing lipid droplet production in a dose-dependent manner. Furthermore, the mRNA expression of adipogenic transcription factors peroxisome proliferator-activated receptor-γ and fatty acid synthetase, CCAAT/enhancer binding protein-α and fatty acid binding protein 4 in 3T3-L1 cells was significantly inhibited by BS treatment. In an in vivo test, the BSadministered group of HFD-induced mice showed less body weight gain, and lower liver and epididymal white adipose tissue weights. The BS-treated mice showed decreased serum levels of leptin and lipids compared to untreated HFD mice and the levels of adiponectin and the HDL-cholesterol/total cholesterol ratio increased. These results indicate that BS inhibits body fat accumulation by reducing the mRNA expression of lipogenesis transcription factors and increasing serum adipokine concentration in in vitro and in vivo tests. Conclusions: BS reduced high fat diet-induced weight gain and had a positive effect on dyslipidemia.
서 언
재료 및 방법
1. 시료추출 및 제조
2. 세포배양 및 분화유도
3. 세포독성 실험
4. Oil-red O 염색
5. 지방관련 유전자 Real-time PCR 분석
6. 동물모델 설정 및 디자인
7. 혈청 및 장기 채취
8. 혈청 생화학 지표 분석 및 adipokine 측정
9. 지방조직 세포의 형태학적 분석
10. 통계처리
결과 및 고찰
1. BS가 지방분화 및 지방구 형성에 미치는 영향
2. BS가 지방분화 관련 유전자 발현에 미치는 영향
3 BS가 비만 동물모델에서 체중변화에 미치는 영향
4. BS가 비만 동물모델에서 간 및 부고환지방에 미치는 영향
5. BS가 비만 동물모델에서 혈중 지질함량에 미치는 영향
6. BS가 비만 동물모델에서 혈중 혈당 및 인슐린, leptin,adiponectin 함량에 미치는 영향
7. 비만 동물모델에서 간 및 지방조직의 병리학적 조직검사
감사의 글
REFERENCES
