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트리메틸틴 처리로 유도된 기억 ·학습 능력 손상 모델에 대한 계피와 금앵자 혼합추출물의 개선 효과 KCI 등재
Ameliorating Effects of Cinnamomum loureiroi and Rosa laevigata Extracts Mixture against Trimethyltin-induced Learning and Memory Impairment Model
pp.353-360
Background: A critical features of Alzheimer’s disease (AD) is cognitive dysfunction, which partly arises from decreased in acetylcholine levels. AD afftected brains are characterized by extensive oxidative stress, which is thought to be primarily induced by the amyloid beta (Aβ) peptide. In a previous study, Cinnamomum loureiroi tincture inhibited acetylcholinesterase (AchE) activity. That study identified AChE inhibitor in the C. loureiroi extract. Furthermore, the C. loureiroi extract enhanced memory in a trimethyltin (TMT)-induced model of cognitive dysfunction, as assessed via two behavioral tests. Rosa laevigata extract protected against oxidative stress-induced cytotoxicity. Administrating R. laevigata extracts to mice significantly reversed Aβ-induced learning and memory impairment, as shown in behavioral tests. Methods and Results: We conducted behavioral to examine the synergistic effects of C. loureiroi and R. laevigata extracts in inhibiting AChE and counteracting TMT-induced learning and memory losses. We also performed biochemical assays. The biochemical results showed a relationship between increased oxidative stress and cholinergic neurons damage in TMT-treated mice. Conclusions: A diet containing C. loureiroi and R. laevigata extracts ameliorated learning and memory impairments in the Y-maze and passive avoidance tests, and exerted synergistic inhibitory effect against AChE and lipid peroxidation.
INTRODUCTION
MATERIALS AND METHODS
1. Materials
2. Sample preparation
3. Cell culture
4. Measurement of AChE activity
5. Animals
6. Y-maze test
7. Passive avoidance test
8. Quantification of ACh content in mice braintissues
9. Determination of lipid peroxidation in mice braintissues
RESULTS AND DISCUSSION
ACKNOWLEDGMENTS
REFERENCES
