Background: Glycyrrhiza uralensis Radix (GR) is a crude drugs used in Asian countries that has been reported to prevent the progression of neurodegenerative diseases such as Alzheimer’s disease. The present study examined whether GR and its active compounds, glycyrrhizic acid (GA) and isoliquiritigenin (IL), exerted protective effects on H2O2-induced oxidative damage in C6 glial cells. Methods and Results: We exposed C6 glial cells to hydrogen peroxide (H2O2) for 24 h and investigated the cellular response to GR and its active compounds by evaluating cell viability, reactivie oxygen species (ROS) production, and apoptosis-related protein expression. GR successfully mitigated the reduced cell viability and ROS production induced by H2O2 in C6 glial cells, IL and GA significantly increased the cell viability and decreased ROS production. In addition, IL and GA down-regulated apoptotic Baxdependent caspase-3 activation, but each compound exerted different mechanisms, i.e., IL dose-dependently decreased ROS production and, GA up-regulated anti-apoptotic Bcl-2 expression. Conclusions: These results demonstrated that GR and its active components, IL and GA, exhibit potential for use as natural neurodegenerative agents for the modulation of apoptosis in C6 glial cells.

서 언
 재료 및 방법
  1. 시료
  2. 세포 종류 및 시약
  3. 세포 배양
  4. 세포 생존율 측정
  5. ROS 소거능 측정
  6. Western Blot Analysis
  7. 통계분석
 결과 및 고찰
 REFERENCES

• 박찬흠(농촌진흥청 국립원예특작과학원 인삼특작부, Department of Herbal Crop Research, NIHHS, RDA) | Chan Hum Park Corresponding author
• 김지현(부산대학교 식품영양학과, 부산대학교 김치연구소, Department of Food Science and Nutrition, Pusan National University, Kimchi Research Institute, Pusan National University) | Ji Hyun Kim
• 최승학(부산대학교 식품영양학과, 부산대학교 김치연구소, Department of Food Science and Nutrition, Pusan National University, Kimchi Research Institute, Pusan National University) | Seung Hak Choi
• 신유수(농촌진흥청 국립원예특작과학원 인삼특작부, Department of Herbal Crop Research, NIHHS, RDA) | Yu Su Shin
• 이상원(농촌진흥청 국립원예특작과학원 인삼특작부, Department of Herbal Crop Research, NIHHS, RDA) | Sang Won Lee
• 조은주(부산대학교 식품영양학과, 부산대학교 김치연구소, Department of Food Science and Nutrition, Pusan National University, Kimchi Research Institute, Pusan National University) | Eun Ju Cho Corresponding author