Hericium erinaceus is considered a functional food and potential medicinal source. The present study was conducted to examine the potential antioxidant and anti-inflammatory activities of carried out with water and ethanol extracts of Hericium erinaceus grown on germinated green rice (HEGR-W and HEGR-E, respectively) and the water and ethanol extracts of germinated green rice (GR-W and GR-E, respectively) as potential medicinal resources or antioxidant and anti-inflammatory agents. Methods and Results: The total phenolic and flavonoid contents, DPPH, and ABTS activity, reducing power, DNA protective activity, cell viability, and NO production were investigated. The total phenolic and flavonoid contents were highest in HEGR-E (66.53 ± 2.40 ㎎·GAE/100 g and 82.12 ± 7.10 ㎎·CE/100 g respectively). HEGR-E exhibited high DPPH (44.70 ± 1.28%) and, ABTS (44.70 ± 1.28%) activity and reducing power (0.219). HEGR and GR extracts showed protective activity against DNA damage. The cytotoxicity of HEGR and GR in RAW264.7 cells and LPS-induced RAW264.7 cells was low. HEGR-E and GR-W exhibited anti-inflammatory effects through a 28% inhibition of NO production in LPS-induced RAW264.7 cells. Conclusions: These results suggested that the extracts of Hericium erinaceus grown on germinated green rice could be a potential medicinal material with natural antioxidant and NO inhibitory properties.

서 언
 재료 및 방법
  1. 실험재료 및 시약
  2. 시료 추출물 제조
  3. 총 폴리페놀 화합물 함량 측정
  4. 총 플라보노이드 화합물 함량 측정
  5. DPPH 라디칼 소거능 측정
  6. ABTS 라디칼 소거능 측정
  7. 환원력 (reducing power) 측정
  8. DNA 보호능 측정
  ９. 세포 배양 (RAW264.7)
  10. 세포 생존률 측정
  11. NO (nitric oxide) 생성량 측정
  12. 통계처리
 결과 및 고찰
  1. 총 폴리페놀 화합물 및 플라보 노이드 함량
  2. DPPH 라디칼 소거능 측정
  3. ABTS 라디칼 소거능 측정
  4. 환원력 측정
  5. 산화적 스트레스에 의한 DNA 손상 억제 효과
  6. MTT assay에 의한 세포 생존율 측정
  7. Nitric oxide 생성 저해효과
 REFERENCES

• 이영민(건국대학교 의생명화학전공, Department of Applied Biochemistry, Konkuk University) | Young Min Lee
• 김인숙(건국대학교 의생명화학전공, Department of Applied Biochemistry, Konkuk University) | In Sook Kim
• 미툰 고쉬(건국대학교 의생명화학전공, Department of Applied Biochemistry, Konkuk University) | Mithun Ghosh
• 홍성민(건국대학교 의생명화학전공, Department of Applied Biochemistry, Konkuk University) | Seong Min Hong
• 이택환((재)경북바이오산업연구원, AG-Health Research Center) | Taek Hwan Lee
• 이동희((재)경북바이오산업연구원, AG-Health Research Center) | Dong Hee Lee
• 임병우(건국대학교 의생명화학전공, Department of Applied Biochemistry, Konkuk University) | Beong Ou Lim Corresponding author