논문 상세보기
pp.290-295
Background: Senna tora is a flowering plant in the legume family Fabaceae. Its seeds are roasted and consumed as tea in Asia, to reduce inflammation in the liver and improve eyesight. Thus, it has been considered as an important medicinal crops in Asia. However, breeding trials to improve its genetic properties are rare. Mutation breeding by gamma ray is known to be an effective and highly successful approach for the generation of agronomically useful cultivars. Here we analyzed the effects of several dosages of gamma ray on the biological conditions of Senna tora seeds. Methods and Results: The germination rate and growth patterns of Senna tora were examined following irradiation with gamma ray at 100, 200, 300 and 400 Gy. The total phenolic compound contents and antioxidant activities of Senna tora were analyzed. Germination increased at 100 and 200 Gy in the M1 and M2 generations compared with that of the control (M0). The total phenolic compound contents and antioxidant activity of the seeds significantly decreased as the radiation dosage increased above 100 Gy in the M1 generation. Conclusions: Senna tora, irradiated with gamma ray at dosages 100, 200, 300, and 400 Gy, showed maximum germination rate at 200 Gy in the M2 generation. Plant height and leaf size gradually decreased with increasing gamma ray intensity in the M2 generation. The total phenolic compound contents decreased significantly at 400 Gy, and the related antioxidant activity was also decreased as the radiation dosage increased.
재료 및 방법
1. 공시재료
2. 발아방법
3. 결명자 종자의 알코올 추출
4. 총 페놀화합물 (total phenolic compounds, TPC) 측정
5. ABTS [2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonicacid)]를 이용한 자유라디칼 소거 활성
6. 통계분석
결과 및 고찰
1. 감마선이 조사된 결명자 종자의 발아율
2. 감마선 조사 결명자의 생육
3. 감마선 조사 후 총 페놀화합물 (total phenolic compounds,TPC) 변화
4. 결명자 추출물의 항산화 활성
REFERENCES
