Background: To investigate the possibility of using Asarum sieboldii as an environment-friendly fumigant for protecting organic cultural heritages, the inhibitory effect of A. sieboldii extract against wood rot fungi on Hanji was examined. Methods and Results: The physical, optical, and morphological properties of Hanji inoculated with Trametes versicolor and Tyromyces palustris, and exposed to the n-hexane fraction of A. sieboldii extract, were measured. The physical properties were expressed as weight loss, zero-span tensile strength and viscosity and the optical properties were depicted by luminance and chromaticity (L*, a*, and b*). The results showed that, the n-hexane fraction of A. sieboldii extract inhibited the growth of fungi on Hanji, and preserved its condition. At a concentration of 25 ㎎, the n-hexane fraction of A. sieboldii extract maintained zero-span tensile strength, increased viscosity, and restricted discoloration of Hanji. It also was confirmed that the weight of fungi infested Hanji exposed to the extract did not decrease. Scanning electron microscopic images revealed that the spores and hyphae of T. versicolor and T. palustris were not present on Hanji during treatment with > 25 ㎎ of the n-hexane fraction of A. sieboldii extract. Conclusions: These results indicate that the n-hexane fraction of A. sieboldii extract by virtue of its antifungal effectiveness may help in preserving Korean paper cultural heritages, including Hanji.

INTRODUCTION
 MATERIALS AND METHODS
  1. Preparation of Hanji
  2. Asarum sieboldii extracts
  3. Fungal strains and Inoculation
  4. Damage assessment of the Hanji
 RESULTS AND DISCUSSION
  1. Effects of A. sieboldii extracts on the physical andoptical properties of Han
  2. Antifungal activities of A. sieboldii extracts on thesurface of Hanji
 REFERENCES

• 홍진영(국립문화재연구소 복원기술연구실, Restoration Technology Division, National Research Institute of Cultural Heritage) | Jin Young Hong
• 김영희(국립문화재연구소 복원기술연구실, Restoration Technology Division, National Research Institute of Cultural Heritage) | Young Hee Kim
• 조창욱(국립문화재연구소 복원기술연구실, Restoration Technology Division, National Research Institute of Cultural Heritage) | Chang Wook Jo
• 이정민(국립문화재연구소 복원기술연구실, Restoration Technology Division, National Research Institute of Cultural Heritage) | Jeong Min Lee
• 김수지(국립문화재연구소 복원기술연구실, Restoration Technology Division, National Research Institute of Cultural Heritage) | Su Ji Kim
• 정소영(국립문화재연구소 복원기술연구실, Restoration Technology Division, National Research Institute of Cultural Heritage) | So Young Jeong Corresponding author