Background: Small particles increase airway inflammation upon reaching the alveoli. Here, we investigated the protective or therapeutic
effects of Salvia plebeia R. Br. (SP_R) extracts on airway inflammation.
Methods and Results: To investigate the anti-inflammatory activity of SP_R extracts, we measured their inhibitory effect on the
production of reactive oxygen species (ROS) expression of inflammatory mediators, and immune cell infiltration in MH-S alveolar
macrophage cells and in the ambient particulate matter (APM)-exposed airway inflammation mice model. The SP_R extracts inhibited
the production of ROS and expression of IL-4, IL-10, IL-15, and IL-17A mRNA in APM-stimulated MH-S cells. Oral administration
of SP_R extracts suppressed APM-induced inflammatory symptoms, such as high alveolar wall thickness, excess collagen
fibers, decreased mRNA expression of chemokines (Ccr9, Ccl5, Ccr3), inflammatory cytokines (IL-15, TNF-α), and IL-4 Th2 cytokine
in the lung. The SP_R extracts also inhibited ROS production, granulocyte (CD11b+Gr-1+) infiltration, IL-17A, TNF-α, macrophage
inflammatory protein (Mip-2), and chemokine (C-X-C motif) ligand 1 (Cxcl-1) production in the airway. The specific
compounds in the SR-R extracts that mediate the anti-inflammatory effects were identified.
Conclusions: In this study, SP_R extracts effectively inhibited airway inflammatory responses, such as ROS production and granulocyte
infiltration into the airway, by regulating the expression of chemokines and inflammatory cytokines.

서 언
 재료 및 방법
  1. 시약 및 재료
  2. 배암차즈기 분리 및 제조
  3. 배암차즈기 HPLC 분석
  4. 미세먼지혼합물 (ambient particulate matter) 제조
  5. Cell culture
  6. MTS cell proliferation assay
  7. DCF-DA cellular reactive oxygen species detectionassay
  8. Real-time reverse transcription polymerase chainreaction (real-time RT-PCR)
  9. Histopathology
  10. Flow cytometric analysis
  11. ELISA (enzyme-linked immunosorbent assay)
  12. 호흡기 손상 동물 모델 제작
  13. 통계분석
 결 과
  1. 항염증 효과를 가진 배암차즈기 추출물의 HPLC 분석
  2. In vitro 실험을 위한 미세먼지의 처리 농도 결정
  3. MH-S 세포에서 배암차즈기 추출물 처리에 따른 세포증식능력과 미세먼지 자극 후 ROS 생성량 분석
  4. 미세먼지에 의해 유도된 호흡기 질환 마우스 모델에서배암차즈기 추출물의 처리에 의한 호흡기 염증 증상 개선
  5. 미세먼지에 의해 유도된 호흡기 질환 마우스 모델의 기관지폐포 세척액에서 ROS 생성량 분석
  6. 미세먼지에 의해 유도된 호흡기 질환 마우스 모델에서 총세포수와 과립구의 population 조사
  7. 미세먼지에 의해 유도된 호흡기 질환 마우스 모델에서chemokine (C-X-C motif) ligand (Cxcl)의 유전자 발현과과립구 수의 상관관계
  8. 미세먼지에 의해 유도된 호흡기 질환 마우스 모델에서배암차즈기 추출물의 처리에 따른 염증관련 케모카인과 사이토카인 조사
 고 찰
 REFERENCE

• 송형우(전남대학교 생물과학·생명기술학부, School of Biological Sciences and Technology, Chonnam National University) | Hyeongwoo Song
• 지건영(전남대학교 생물과학·생명기술학부, School of Biological Sciences and Technology, Chonnam National University) | Kon Young Ji
• 김복규(대전대학교 동서생명과학연구원, Institute of Traditional Medicine and Bioscience, Daejeon University) | Bok Kyu Kim
• 양원경(대전대학교 동서생명과학연구원, Institute of Traditional Medicine and Bioscience, Daejeon University) | Won Kyung Yang
• 한창균(KGC 중앙연구원, KT&G Research Institute) | Chang Kyun Han
• 신한재(KGC 중앙연구원, KGC Research Institute) | Han Jae Shin
• 박양춘(대전대학교 폐계내과학교실, Division of Respiratory System, Department of Internal Medicine, Daejeon University) | Yang Chun Park
• 황지숙(대전대학교 폐계내과학교실, Division of Respiratory System, Department of Internal Medicine, Daejeon University) | Ji Sook Hwang
• 강형식(전남대학교 생물과학·생명기술학부, School of Biological Sciences and Technology, Chonnam National University) | Hyung Sik Kang
• 김승형(대전대학교 동서생명과학연구원, Institute of Traditional Medicine and Bioscience, Daejeon University) | Seung Hyung Kim Corresponding author