This study was carried out to develope mushroom growing medium for Pleurotus eryngii cultivation using persimmon peels. Total nitrogen, carbon source and protein of commercial cultivation medium used in this study was 1.34, 53.9 and 0.09 %, respectively. The C/N ratio was 40.22. The total nitrogen was increased by increasing mixing ratio of persimmon peels. The carbon source and protein was no significant difference by increasing mixing ratio of persimmon peels. In column test, mycelial growth of 15% persimmon peels treatment was similar with control, but mycelia of 20-50% persimmon peels treatment was growed slowly compared with control. To investigate yields of fruiting body, 15% of persimmon peels was mixed with commercial cultivation medium. The fruiting body of 15% persimmon peels treatment was no significant difference yield compared with control. But, the chemical contents of 15% persimmon peels treatment were the higher than control.